Elevated Lipoprotein(a) levels dictate a unique plaque phenotype and transcriptomic signature in transgenic mice with advanced atherosclerosis

ABSTRACT: We assessed the sex-specific effects of elevated Lp(a) on the development and progression of atherosclerosis in a humanized mouse model. Wild-type and mice with a humanized LPA gene were subject to CRISPR-mediated Ldlr knock out and 24 weeks of high-fat, high-cholesterol diet to induce atherosclerosis. Diseased aortic cells were dissociated and pooled (2 animals per pool) then sorted for live-dead and subject to 3' single-cell RNAseq using 10X Chromium V3 chemistry. We identified significant changes in macrophage phenotype with an increase in contractility markers suggesting enhanced myeloid-myofibroblast transition in LPA+ mice.

INSTRUMENT(S): NA, 10X Chromium Next GEM Single-Cell 3' Kit v3.1, Illumina NovaSeq X, AAV-CRISPR, Digital Alliance of Canada, 10X Dual Index Kit

ORGANISM(S): Mus musculus

SUBMITTER: Tallulah Andrews 

PROVIDER: E-MTAB-17061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress