HmU-Seq Reveals Genome-Wide Distribution of 5-Hydroxymethyluracil via Selective Enzymatic and Chemical Labeling

ABSTRACT: 5-Hydroxymethyluridine (5hmU) is an epigenetic thymine modification and occurs across diverse organisms. 5hmU has been linked to cancer development and therapy resistance, yet its precise biological functions remain poorly understood. Genomic profiling of 5hmU is severely hindered by cross-reactivity with 5-hydroxymethylcytosine (5hmC), producing false positives that confound biological interpretation and prevent robust genome-wide mapping in higher eukaryotes. To address these limitations, we present hmU-seq, a highly specific enzymatic–chemical labelling and capture method for genome-wide 5hmU profiling. We validated the robustness and sensitivity of hmU-seq by mapping 5hmU dynamics across two life-cycle stages of Trypanosoma brucei. We then applied hmU-seq to mouse embryonic stem cells

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Marlon Steven Zambrano Mila 

PROVIDER: E-MTAB-17088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress