Fusion gene KMT2A::SEPTIN6 in AML cell line KOPM-88

ABSTRACT: KMT2A (alias MLL) is located at chromosomal position 11q23 and en-codes histone methyltransferase 2A which activates genes via methylation of histone H3 lysine K4 in their chromatin. Mutations of KMT2A, including partial tandem duplication (PTD) and fusion with partner genes are present in both lymphoid and myeloid acute leukemia. More than 100 different KMT2A fusion genes are described while only some of them are represented in cell line models. Cytogenetic and genomic copy number analyses, PCR, Western blot and RNA-sequencing were performed to characterize aberrations in acute myeloid leukemia (AML) cell line KOPM-88. Bioinformatic analysis of public AML patient data revealed differentially expressed genes. Functional analyses were performed in KOPM-88 by siRNA-mediated knockdown and lif

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Claudia Pommerenke 

PROVIDER: E-MTAB-17091 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress