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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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GBM-Space: Joint Transcriptome and Chromatin Accessibility Profiling of Glioblastoma (10x Genomics - Multiome)

ABSTRACT: Cancer cells display highly heterogeneous and plastic states in glioblastoma, an incurable brain tumour. However, how these malignant states arise and whether they follow defined cellular trajectories across tumours is poorly understood. Here, we generated a deep single cell and spatial multi-omic atlas of human glioblastoma that pairs transcriptomic, epigenomic and genomic profiling of 12 tumours across multiple regions. Please cite De Jong et al., bioRxiv 2025 upon use. This submission contains the Cell Ranger ARC processed outputs from single nuclei joint transcriptome- and chromatin accessibility-sequencing (10x Genomics) for each multiome reaction generated as part of the GBM-space atlas. See README_cellranger_arc.md We also provide processed and annotated 10x multiome snRNA-seq and snATAC-seq objects across the dataset. See GBM_space_snRNA_README.md and GBM_space_ATAC_filtered_peaks_README.md respectively. Finally, we provide metacell-level aggregated snRNA-seq expression and snATAC-seq gene-activity scores derived from our GBM-space atlas, used for the epigenetic plasticity analysis in Saraswat M et al., bioRxiv 2025. See README_metacell.md.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Oliver Gould

PROVIDER: E-MTAB-17183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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