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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ScRNAseq NOHA

ABSTRACT: To investigate the impact of hormonal modulation on neurodevelopmental processes, we performed single-cell RNA sequencing (ScRNA-seq) on neural organoids derived from two genetically validated human induced pluripotent stem cell (hiPSC) control lines (CTL08, male; CTL04, female). Organoids were chronically exposed to agonists and inhibitors targeting seven hormone signaling pathways known to regulate human brain development: androgen (AND), estrogen (EST), glucocorticoid (GC), thyroid (THY), retinoic acid (RA), liver X (LX), and aryl hydrocarbon (AH). Appropriate negative controls were included, consisting of vehicle-treated (DMSO) and/or untreated samples depending on the experimental condition. GitHub: https://github.com/GiuseppeTestaLab/noha Cellxgene: https://cellxgene.cziscience.com/collections/2bebc4ae-69a2-4001-9d2f-091ec9b4020c

INSTRUMENT(S): Illumina NovaSeq 6000, Illumina NOVAseq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Alessia Valenti

PROVIDER: E-MTAB-17184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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