Illumina Short-read NGS of a de novo RNA hairpin library using TInDel Assembly
Ontology highlight
ABSTRACT: A library of hairpins was generated using TInDel Assembly to be tested for RNA thermometer activity. This method provides variation in sequence, length, and topology at the RNA level. The library was generated in vitro and cloned into an expression vector for screening. The library was amplified via PCR from the vector for submission for NGS.
INSTRUMENT(S): Illumina MiSeq
ORGANISM(S): Escherichia coli
SUBMITTER: Rosa-Maria Määttälä
PROVIDER: E-MTAB-17203 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA