Pathogenic Keratinocyte States and Fibroblast Niches Define the Tissue Microenvironment in Severe Hidradenitis Suppurativa

ABSTRACT: Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease characterized by recurrent painful abscesses and tunnels in flexural sites. The mechanisms driving HS pathogenesis, particularly the interactions between epithelial, stromal and immune cell populations, remain incompletely understood. To characterise the cellular and molecular landscape of HS, we analyzed lesional skin from severe HS patients using single-cell RNA-sequencing and spatial transcriptomics, with a focus on fibroblast-keratinocyte interactions. We identified a migratory S100+ pathogenic keratinocyte state enriched within HS lesions and observed spatially distinct fibroblast populations associated with different tissue compartments. COL6A5+ papillary fibroblasts showed spatial association with undifferentiated

INSTRUMENT(S): Bio-Rad CFX96 Real-time System, Epidermal cells : Trypsin dissociation / Dermal cells :MACS Whole Skin Dissociation Kit, BD LSRFortessa, Illumina HiSeq 4000, Advanced Sequencing Facility

ORGANISM(S): Homo sapiens

SUBMITTER: Clarisse Ganier 

PROVIDER: E-MTAB-17238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress