RNA-seq expreiment of a striatin knockout mutant compared to wildtype Schyzophyllum commune

ABSTRACT: This study focused on the lipid raft-associated protein striatin. In filamentous fungi, striatin has been implicated in fruiting body development and in the regulation of sexual development, vegetative growth, and hyphal fusion. In Schizophyllum commune, striatin primarily regulates cell fusion and multicellular differentiation during sexual development. To investigate its precise role in S. commune, a homozygous knockout mutant (∆str1) was generated. For this study, the dikaryotic and monokaryotic life stages of the ∆stri mutant and the wild type were cultivated, and gene expression differences between them were analyzed using RNA sequencing (∆str1 vs. wild type). Schizophyllum commune wild type and the ∆str1 mutant were inoculated in triplicate on CYM medium (Complex Yeast Medium; Schwalb and Miles, 1967) and incubated for 6 days. For RNA isolation and subsequent RNA sequencing, the fungal mycelium was harvested and ground to a fine powder under liquid nitrogen. Subsequently, 100 mg of frozen tissue was used for RNA extraction with the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. RNA concentration and quality were assessed spectrophotometrically using a DeNovix DS-11 (Wilmington, USA). RNA samples were submitted to StarSEQ GmbH (Mainz, Germany) for transcriptome analysis.

INSTRUMENT(S): RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), NEBNext Ultra II Directional RNA Library Prep Kit, NextSeq 2000

ORGANISM(S): Schizophyllum commune

SUBMITTER: Berit Frizzy Porsche 

PROVIDER: E-MTAB-17277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress