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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of mixed glial cultures grown on different growth surfaces

ABSTRACT: Biomechanical alterations of the extracellular matrix (ECM) and injury-induced or disease-induced cell loss are major contributors to pathological astrocyte activation (astrogliosis) in the CNS and the formation of glial scars. This in vitro experiment is intended to identify transcriptional alterations in mixed glial cultures grown on surfaces of different stiffness (rigid, 650 Pa hydrogel, 250 Pa hydrogel) and also in a confluent mixed glial monolayer on a stiff surface after injury by scratching with a plastic pipette tip.100,000 mixed glial cells were plated on different growth surfaces. After adhesion, confluent monolayers on different hyaluoronic acid/gelatin rigid or hydrogel surfaces were grown in low serum (0.5 % FBS) media for 48 h before RNA extraction. Adherent confluent monolayers on rigid PDL-coated surfaces were left uninjured or scratch-injured and were grown in low serum (0.5 % FBS) media for 48 h before RNA extraction.

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Mus musculus

SUBMITTER: Emily Brockmann

PROVIDER: E-MTAB-17287 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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