Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Pacbio Hifi sequencing of a naked mole-rat male subordinate kidney sample

ABSTRACT: Two PacBio Hifi sequencing runs from the kidney of a single male NMR sample used to make the mHetGla4.1.primary genome assembly. Specifically, we assembled a second NMR genome from an unrelated male of a separate captive colony in Toronto, Canada, using PacBio HiFi (155.8 Gb, read N50 = 11.45 Kb) and ONT-LSK (299.6 Gb, read N50 = 10.1 Kb) reads (contig N50 = 75.7 Mb, Compleasm S = 98%). This accession stores the Pacbio Hifi data for this independent assembly.

INSTRUMENT(S): PacBio RS II

ORGANISM(S): Heterocephalus glaber

SUBMITTER: Dustin Sokolowski 

PROVIDER: E-MTAB-17313 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An updated reference genome sequence and annotation reveals gene losses and gains underlying naked mole-rat biology.

Sokolowski Dustin J DJ   Miclăuș Mihai M   Nater Alexander A   Faykoo-Martinez Mariela M   Hoekzema Kendra K   Zuzarte Philip P   Monis Simon S   Alvi Sana Akhtar SA   Erdmann Jason J   Erdmann Archana Lal AL   Kumaragurubaran Rathnakumar R   Bayerl Jonathan J   Yoo DongAhn D   Karimpour Nadia N   Ungerleider Kyra K   Hou Huayun H   J Martin Fergal F   Hourlier Thibaut T   Clarke Zoe Z   Lischer Heidi E L HEL   Leordean Dragos V DV   Jiang Yiyue Y   Pugh Trevor J TJ   Smith Ewan St J ESJ   Haggerty Leanne L   Laird Diana J DJ   Lilue Jingtao J   Holmes Melissa M MM   Eichler Evan E EE   Bruggmann Rémy R   Simpson Jared T JT   Balmus Gabriel G   Wilson Michael D MD  

bioRxiv : the preprint server for biology 20241128

The naked mole-rat (NMR; <i>Heterocephalus glaber</i>) is a eusocial subterranean rodent with a highly unusual set of physiological traits that has attracted great interest amongst the scientific community. However, the genetic basis of most of these traits has not been elucidated. To facilitate our understanding of the molecular mechanisms underlying NMR physiology and behaviour, we generated a long-read chromosomal-level genome assembly of the NMR. This genome was subsequently annotated and in  ...[more]

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