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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of T cells form house dust mite mouse model of bronchoalveolar lavage (BAL), medLN and lung tissues

ABSTRACT: This dataset profiles T cells recovered from the airway lumen (bronchoalveolar lavage, BAL), medLN and lung tissues of mice exposed to house dust mite (HDM) allergen, a model of allergic airway inflammation. The aim of this experiment was to capture, at single-cell resolution, both the transcriptional states and the T cell receptor sequences (TCR) to identify T cell clones in the airway . The BAL data serve as an in vivo application of PhyloTrajectory and used to compare inferred trajectories with Dog Allergen model. Mouse was exposed to HDM. T cells (CD4+ CD3+ B220−) were isolated from bronchoalveolar lavage by FACS, and processed for single-cell RNA sequencing with paired TCR (VDJ) profiling using 10x Genomics Chromium.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Mariia Guryleva

PROVIDER: E-MTAB-17402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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