3' sequencing following PRMT inhibition in RPE-1 cells and CDK4/CDK6 inhibition in LU-99 cells

ABSTRACT: Numerous RNA-binding proteins are arginine methylated and inhibition of arginine methylation triggers changes in alternative polyadenylation in cancer cells. Therefore we aimed to investigate whether this phenotype was also triggered in an untransformed cell line - RPE-1. RPE-1 cells were treated with 100 nM LLY-283 to inhibit symmetric dimethylation and 3 μM GSK-715 to inhibit asymmetric dimethylation for 72 hours. We also investigated whether treatment of a lung cancer line - LU99 - with another compound that causes reduced proliferation - Palbociclib - also triggers similar alternative polyadenylation as arginine methylation inhibition. In this case, LU-99 cells were treated with 1 μM Palbociclib for either 24 or 48 hours. We sequenced the 3' ends of poly-adenylated mRNAs extracted from

INSTRUMENT(S): N/A, Maxwell RSC Instrument, Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Jernej Ule 

PROVIDER: E-MTAB-17463 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress