3' sequencing following arginine methylation inhibition +/- FLAG-PCF11, FLAG-CSTF2 or FLAG-GFP in LU-99

ABSTRACT: Cleavage and polyadenylation factors PCF11 and CSTF2 are arginine methylation substrates. Therefore we aimed to study how the alternative polyadenylation changes caused by arginine methylation inhibition are affected by overexpression of either PCF11 or CSTF2. We generated LU-99 cell lines expressing FLAG-PCF11, FLAG-CSTF2 or FLAG-GFP under the control of a doxycycline-inducible promoter. We treated these cells with 100 nM LLY-283 to inhibit symmetric dimethylation and 3 μM GSK-715 to inhibit asymmetric dimethylation for 48 hours, with or without doxycycline-induced expression of these FLAG-tagged proteins. We sequenced the 3' ends of poly-adenylated mRNAs extracted from these cells.

INSTRUMENT(S): N/A, Maxwell RSC Instrument, Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Jernej Ule 

PROVIDER: E-MTAB-17496 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress