Microbiome‐Based Modeling of CAR‐T Therapy Response in Lymphoma: Insights From Shotgun Metagenomics Sequencing

ABSTRACT: The interplay between the commensal microbiota and the mammalian immune system may influence the outcomes of T cell‐driven cancer immunotherapies. However, clinical studies supporting microbiota‐based interventions in chimeric antigen receptor T‐cell (CAR‐T) therapy remain scarce. This study included 30 adult patients with B‐cell lymphoma treated with axicabtagene ciloleucel (axi‐cel) or 4‐1BB investigational product. Shotgun metagenomics sequencing (SMS) of fecal samples, collected before lymphodepletion and 1 month post infusion, enabled species‐level resolution. We also trained 25 microbiome‐based machine‐learning (ML) models for response prediction. Neither prior “high‐risk” antibiotics exposure nor alpha diversity influenced toxicity, response, or survival. However, dysbiosis was obse

INSTRUMENT(S): NextSeq 550

ORGANISM(S): human gut metagenome

SUBMITTER: Sheila Zúñiga Trejos 

PROVIDER: E-MTAB-17540 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress