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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Bacillus cereus ATCC14579 and its isogenic SecDF deletion mutant

ABSTRACT: SecDF is a highly conserved accessory protein of the Sec-translocase located in the cytoplasmic membrane. The deletion mutant (delta Bc4405) of Bacillus cereus ATCC14579 shows multiple phenotypic changes, including aberrent cell division starting at transitionary phase. To understand the underlying processes genotypic profiling was carried out at 3h and 4h after inoculation. The morphology of the mutant seems to be more severe if glucose is added to the LB medium, thus all cultures contained 1% glucose.

ORGANISM(S): Bacillus cereus ATCC 14579

SUBMITTER: Aniko Voereos

PROVIDER: E-MTAB-1759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.

Vörös Aniko A Simm Roger R Slamti Leyla L McKay Matthew J MJ Hegna Ida K IK Nielsen-LeRoux Christina C Hassan Karl A KA Paulsen Ian T IT Lereclus Didier D Økstad Ole Andreas OA Molloy Mark P MP Kolstø Anne-Brit AB

PloS one 20140801 8

The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less a ...[more]

PMID: 25083861

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