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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ScRNA-seq of lymph node of mice where Cip2a locus has been disrupted and wild-type control mice at the peak of Experimental Autoimmune Encephalomyelis

ABSTRACT: We induced EAE in mice carrying a gene-trap disruption of Cip2a (hereafter referred to as knockout [KO] mice) and wild-type (WT) control C57BL/6J male and female mice, aged 8-12 weeks. We performed scRNA-seq analysis of lymph node cells from five WT and five KO mice at the peak of EAE. The goal was to see if there are differences in cell type abundance and gene expression between WT and KO mice. The samples were sequenced in two technical replicates, rep1 and rep2. Using cell multiplexing oligo (CMO) by 10X, 5 KO mice lymph nodes (CMO301-305) and 5 WT tagged (CMO306-310) processed as 1 sample for 10X chromium. Same sample ran twice on two lanes of Chromium chip as technical replicate for capturing more single cells for analysis.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Ubaid Ullah Kalim

PROVIDER: E-MTAB-17644 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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