ArchR projects from single-nucleus ATAC-seq datasets across 4 Poeciliid fishes

ABSTRACT: Characterisation of the chromatin accessibility of cell types in the ovary of livebearing Poeciliid species. Fish were culled by anaesthetic overdose (MS222 or Aquased) followed by decapitation under local animal use regulations, with procedures approved by the local ethics committees at each institution. The maternal follicular wall, yolk sac, whole ovaries and livers were then recovered by dissection. The MFW was released by gripping the tissue with two pairs of forceps and gently pulling them apart to tear it, creating a gap to remove the embryo, the yolk sac was then subsequently detached by pinching at the yolk sac–embryo boundary with one pair of forceps and pulling the embryo away with the other. Single nuclei were isolated to prepare single-nuclei libraries. Libraries were generate

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Poeciliopsis gracilis

SUBMITTER: Sophie Kraunsoe 

PROVIDER: E-MTAB-17776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress