Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

17 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

JHI St WM daylength

ABSTRACT: Recent advances have defined some of the components of photoperiodic signalling that lead to tuberisation in potato including orthologues of FLOWERING LOCUS T (StSP6A) and CYCLING DOF FACTOR (StCDF1). The aim of the current study is to investigate the molecular basis of permissive tuber initiation under long days in Solanum tuberosum Neo-Tuberosum by comparative analysis with an obligate short day Solanum tuberosum ssp. Andigena accession. We show that the Neo-Tuberosum accession, but not the Andigena, contains alleles that encode StCDF1 proteins modified in the C-terminal region, likely to evade long day inhibition of StSP6A expression. We also identify an allele of StSP6A from the Neo-Tuberosum accession, absent in the Andigena, which is expressed under long days. Other leaf transcripts and metabolites that show different abundances in tuberising and non-tuberising samples were identified adding detail to tuberisation-associated processes. Overall, the data presented in this study highlight the subtle interplay between components of the clock-CONSTANS-StSP6A axis which collectively may interact to fine-tune the timing of tuberisation.

ORGANISM(S): Solanum tuberosum

SUBMITTER: Pete Hedley

PROVIDER: E-MTAB-1799 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Characterization and detection of novel and conserved miRNAs in Potato

Project description:small RNA profile of Solanum tuberosum group Andigena small RNA profile of Solanum tuberosum group Andigena

2013-04-01 | E-GEOD-39230 | biostudies-arrayexpress

Transcriptional profiles associated with altered StCEN expression in non-swelling stolons from RNAi and overexpressing potato (subspecies Andigena) transgenic lines demonstrating altered tuberization phenotypes

Project description:Two independent Solanum tuberosum ssp. Andigena ADG StCEN RNAi and two ADG StCEN 35S overexpressing OE transgenic lines and a wild type WT control were grown under standard glasshouse conditions for 6 weeks prior to being moved to controlled growth cabinet conditions. Plants were grown under 4 different daylengths (8,10,12 and 16 hr) at 20 °C day and 14 °C night temperatures. ADG StCEN RNAi lines showed signs of early tuberization phenotype compared with the WT control after 23 days in the cabinets. Non-swelling stolons were harvested at this timepoint. ADG StCEN 35S OE lines demonstrated a delayed tuberization phenotype compared with the WT control. Non-swelling stolons were harvested after 38 days in the controlled cabinet conditions.

2020-07-01 | E-MTAB-8346 | biostudies-arrayexpress

Characterization and detection of novel and conserved miRNAs in Potato

Project description:small RNA profile of Solanum tuberosum group Andigena

2013-04-01 | GSE39230 | GEO

EST mining of yellow potato and gene expression profiling of its infection by P. infestans using SAGE - SOLEXA

Project description:The late blight pathogen, Phytophthora infestans has a broad host range within the Solanaceae family, including yellow potato (Solanum phureja). The disease caused by P. infestans in S. phureja is poorly understood and is a major concern in Colombia. Expressed Sequence Tag (EST) libraries obtained from a normalized library constructed from healthy plant tissue revealed high levels of sequence similarity between S. phureja and S. tuberosum. Then, utilizing Serial Analysis of Gene Expression and high-throughput sequencing (SAGE-Solexa), we characterized yellow potato gene expression during infection by P. infestans. Four-week-old yellow potato plants were inoculated with P. infestans and were collected at 12 and 72 hours post inoculation for RNA extraction. We detected differentially expressed genes by comparing inoculated to non-inoculated and resistant to susceptible plants. The discovery and characterization of the proteins mediating this host–pathogen interaction enable the understanding of the pathosystem and is the key for developing resistant plants. Keywords: SAGE-Solexa, inoculation response, transcript profiling, Solanum phureja, Phytophthora infestans Four-week-old yellow potato (Solanum phureja) plants were inoculated with Phytophthora infestans and were collected and flash frozen in liquid nitrogen at 12 and 72 hours post inoculation, as well as mock inoculated, for RNA extraction. 2 yellow potato cultivars (resistant and susceptible) were used for each experiment. Mock inoculated plants were collected in each replicate. RNA obtained from each of the three biological replicates was pooled to obtain a single RNA sample for each timepoint X cultivar combination. A total of 6 different SAGE libraries were thus obtained. For all libraries, Illumina sequencing was performed at Canada´s Michael Smith Genome Sciences Centre.

2009-06-13 | E-GEOD-11781 | biostudies-arrayexpress

Transcriptional changes during photoperiodic tuber induction in Solanum tuberosum ssp andigena

Project description:For expression profiling analyses of early stages of tuber induction, plants of Solanum tuberosum ssp andigena (7540) were used. This wild subspecies is strictly dependent on photoperiod for tuberisation, such that short days (SD) inductive conditions are required in order to trigger tuber induction in the stolons. Andigena plants were grown in the greenhouse under LD non-inductive conditions until a 10-leaf stage. They were subsequently transferred to inductive SD conditions (8 h light/16 h dark), and sampled at 0, 2, 4, 6 and 8 days after transfer to SDs. Tuber swelling was visible approximately 6-8 days after transfer to inductive conditions. The apical region of the stolons (2 cm) was collected one hour before the beginning of the light period. All collected stolons (LD; and 2d, 4d, 6d and 8d under SD) were processed as described. Hybridization scheme with a loop design was applied to compare one sample against the rest of the samples in the induction time course.

2011-10-05 | E-GEOD-31336 | biostudies-arrayexpress

Transcriptomics of shade avoidance and drought in Solanum tuberosum.

Project description:The intent was to study, from transcriptome analysis, shade and drought responses in Solanum tuberosum (potato). We performed Illumina 50 bp single-end RNA-seq in tissues of control and treated var. Spunta wild-type plants. Drought experiments also included two independent AtBBX21-overexpressing (BBX21-OE) potato lines.

2023-07-12 | E-MTAB-12771 | biostudies-arrayexpress

Gene expression study after 3 days of cold or salt exposure in a Solanum tuberosum cultivar called Desirée

Project description:This SuperSeries is composed of the following subset Series: GSE34110: Gene expression study after 3 days of cold exposure in a Solanum tuberosum cultivar called Desirée GSE34116: Gene expression study after 3 days of salt exposure in a Solanum tuberosum cultivar called Desirée Refer to individual Series

2011-12-03 | E-GEOD-34118 | biostudies-arrayexpress

Solanum tuberosum cultivar:andigena

Project description:Solanum tuberosum cultivar:andigena Raw sequence reads

| PRJNA301499 | ENA

Effects of StSP6A overexpression and silencing on potato stolons

Project description:Summary StSP6A is a protein with sequence homology to the Arabidopsis FLOWERING LOCUS T (FT). FT is a main component of the long range flowering promoting signal or florigen that induces photoperiodic flowering under long days (LDs). In potato, short days (SDs) promote tuber induction, and wild subspecies like Solanum tuberosum ssp andigena (wild type line 7540) are strictly dependent on SDs to tuberize. StSP6A transcription is induced in leaves and stolons at early stages upon transfer to SD inductive conditions, priotr to visible solon swelling. Transgenic andigena lines over-expressing the StSP6A gene under the control of the 35S promoter are able to tuberize under LD non-inductive conditions, whereas silencing of this gene by means of an specific RNAi construct are strongly delayed in tuber induction in SD inductive conditions. This data indicate that StSP6A plays a central role in the control of tuber induction. For each sample of the two comparisons (35S-StSP6A versus Wild Type and StSP6A-RNAi versus Wild Type) two biological replicates were prepared. In the case of 35S-StSP6A and StSP6A-RNAi each replicate was composed by a pool of several transgenic lines.

2011-10-05 | E-GEOD-31178 | biostudies-arrayexpress

Transcriptional changes during photoperiodic tuber induction in Solanum tuberosum ssp andigena

2011-10-06 | GSE31336 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data