Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Salmonella 14028s AraC ChIP-Seq

ABSTRACT: We mapped the genome-wide binding of C-terminally FLAG-tagged AraC in S. enterica subsp. enterica serovar Typhimurium strain 14028s using ChIP coupled with deep sequencing (ChIP-seq). We identified five putative target loci for AraC: upstream of araB/araC, araE, araJ, STM14_0178, and within sseD.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S

SUBMITTER: Joseph Wade 

PROVIDER: E-MTAB-1915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-scale analyses of Escherichia coli and Salmonella enterica AraC reveal noncanonical targets and an expanded core regulon.

Stringer Anne M AM   Currenti Salvatore S   Bonocora Richard P RP   Baranowski Catherine C   Petrone Brianna L BL   Palumbo Michael J MJ   Reilly Andrew A AA   Zhang Zhen Z   Erill Ivan I   Wade Joseph T JT  

Journal of bacteriology 20131122 3

Escherichia coli AraC is a well-described transcription activator of genes involved in arabinose metabolism. Using complementary genomic approaches, chromatin immunoprecipitation (ChIP)-chip, and transcription profiling, we identify direct regulatory targets of AraC, including five novel target genes: ytfQ, ydeN, ydeM, ygeA, and polB. Strikingly, only ytfQ has an established connection to arabinose metabolism, suggesting that AraC has a broader function than previously described. We demonstrate  ...[more]

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