MNase and HpaII sensitivity assays in MCF7 breast cancer cells

ABSTRACT: DNA are packaged into nucleosomes and chromatin. We performed incomplete MNase digestion of chromatin to identify nucleosome-free regions that may indicate active promoters and regulatory regions. Additionally, HpaII digestion was performed which cleaves CCGG sites when the internal C remains unmodified. The hypomethylation state and nuclease sensitivity of the chromatin are indicators of transcription regulatory regions.

INSTRUMENT(S): 454 GS 20, Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Ming-Ta Hsu 

PROVIDER: E-MTAB-1958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress