Transcription profiling of Legionella pneumophila letS insertion mutants

ABSTRACT: L. pneumophila Lp02 (thyA hsdR rpsL; MB110) is a virulent thymine auxotroph derived from the Philadelphia 1 clinical isolate and was the parental strain for all constructed mutants. To construct a letS (lpg1912) insertion mutant that lacks pflaG, the letS locus containing the transposon insertion was amplified from MB417 and transferred to Lp02 by natural competence, resulting in strain MB416 (27). Within each experiment, similar culture densities were used to analyze strain phenotypes.

ORGANISM(S): Legionella pneumophila

SUBMITTER: Matthieu JULES 

PROVIDER: E-MTAB-200 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress