HL_Arabidopsis_ost1-1

ABSTRACT: Arabidopsis thaliana plants from the genotypes Ler and ost1-1 (knock-out) were used. They were grown in a growth chamber for 5 weeks in short day (8/16 light/dark cycle) at 22 degrees, 150 umol*m2*s-1 (fluorescent lights) and 55% RH in 100-ml pots with Levingtons L2+S. On the day of the experiment, two hours after the lights turned on they were subjected to mild light stress (approx. 1500 umol*m-2*s-1, measured with a PAR light meter) for 30 min under an Isolight machine (white LED array), and immediately snap-frozen in liquid nitrogen. The outer leaves from four biological replicates were ground and RNA purified, and labelled RNA was produced with the Agilent Quick Amp kit. Labelled RNA was hybridized to Agilent Arabidopsis V4 microarray slides, and the fluorescence recorded with a Genepi

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ruben Alvarez-Fernandez 

PROVIDER: E-MTAB-2051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress