Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

7 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Leukocyte-subset-methylation

ABSTRACT: Genome-wide methylation patterns observed in primary human leukocyte subsets were used to identify cell-typeM-^Vspecific regulatory hypomethylated regions. To investigate the possible effect of these regions on gene expression, a transcriptomics analysis was performed with mRNA isolated from the leukocyte subsets.

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Coulson

PROVIDER: E-MTAB-2062 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Immune transcriptional profiling in end-stage renal disease

Project description:Purified leukocyte subsets in patients with end-stage renal disease were compared transcriptionally with those from healthy controls

2018-01-11 | E-MTAB-2413 | biostudies-arrayexpress

naive-cd25 profiling

Project description:Naive T cell subsets in humans defined by CD25 expression

2013-02-21 | E-MTAB-1138 | biostudies-arrayexpress

Type-I interferon response profiling

Project description:Peripheral blood mononuclear cells were treated with beta-interferon for 2, 6 and 18 hours, and interferon-responsive genes identified. This 'signature' was then analysed in newly diagnosed and long-standing type-1 diabetics, as well as pre-diabetic children and individuals with systemic lupus erythematosus.

2014-03-13 | E-MTAB-1724 | biostudies-arrayexpress

Mouse miR-210 activity in B-cells

Project description:mRNA profiling of miR-210 transgenic (in vivo), mimic-transfected (in vitro) and miR-210 knockout activated mouse B-cells was performed to assess the effect of miR-210 overexpression on the B-cell transcriptome.

2013-08-23 | E-MTAB-1758 | biostudies-arrayexpress

K450 arrays leukocyte subsets

Project description:DNA methylation profiles of primary human leukocyte subsets. Complementary MeDIP-seq data for the same samples have been deposited in ArrayExpress under accession number E-ERAD-179 (https://www.ebi.ac.uk/arrayexpress/experiments/E-ERAD-179/) .

2013-12-09 | E-MTAB-2145 | biostudies-arrayexpress

Leukocyte subset-specific type 1 interferon signatures in SLE and other immune-mediated diseases.

Project description:Objectives Type 1 interferons (IFN-I) are implicated in the pathogenesis of systemic lupus erythematosus (SLE), but most studies have only reported the effect of IFN-I on mixed cell populations. We aimed to define modules of IFN-I associated genes in purified leukocyte populations and use these as a basis for a detailed comparative analysis. Methods CD4+ and CD8+ T-cells, monocytes and neutrophils were purified from patients with SLE, other immune-mediated diseases and healthy volunteers (HV)and gene expression determined by microarray. Modules of IFN-I associated genes were defined using weighted gene coexpression network analysis. The composition and expression of these modules was analysed. Results 1,150 of 1,288 IFN-I associated genes were specific to myeloid subsets, compared with 11 genes unique to T-cells. IFN-I genes were more highly expressed in myeloid subsets compared to T-cells. A subset of neutrophil samples from HV and conditions not classically associated with IFN-I signatures displayed increased IFN-I gene expression, whereas upregulation of IFN-I associated genes in T-cells was restricted to SLE. Conclusions Given the broad upregulation of IFN-I genes in neutrophils including in some HV, investigators reporting IFN-I signatures on the basis of whole blood samples should be cautious about interpreting this as evidence of bona fide IFN-I mediated pathology. Instead, specific upregulation of IFN-I associated genes in T-cells may be a useful biomarker and a further mechanism by which elevated IFN-I contributes to autoimmunity in SLE.

2016-06-01 | E-MTAB-2713 | biostudies-arrayexpress

Transcription profiling of human major leukocyte types isolated or differentiated and stimulated by LPS, IgE, and PMA from blood to characterise their expression profiles

Project description:WArning Files GSM90663.txt and GSM90665.txt are identical. In the present study we used Affymetrix oligonucleotide microarrays to produce gene transcription profiles for the major leukocyte types in humans. This comprehensive dataset enabled us to not only establish which genes were expressed in each leukocyte type, but also which genes were expressed in each subset after activation. The used of a comprehensive dataset of gene profiles from all the major human leukocyte subsets enabled a novel and powerful means for identification of genes associated with single leukocyte subsets, or different immune paradigms. Experiment Overall Design: Different leukocyte subsets were either isolated or differentiated from human blood to obtain consistently >95% pure populations. Gene profiles for activated effector cells such as macrophages, neutrophils and mast cells were also generated. In most cases, microarray analyses were perform on samples from 2 independent donors.

2007-10-27 | E-GEOD-3982 | biostudies-arrayexpress

Purified human leukocyte subtype DNA methylation profiles

Project description:The objective of the study was to identify differentially methylated regions of DNA (DMRs) that distinguish human leukocyte subtypes, and hence serve as biomarkers for those immune cell types. This file contains Illumina Infinium HumanMethylation27 BeadChip data for human leukocyte subtypes that were purified from whole blood samples via magnetic activated cell sorting (MACS) and purity confirmed by flourescence activated cell sorting (FACS).

2012-11-05 | GSE39981 | GEO

Leukocyte gene expression in a model of perceived social isolation (PSI)

Project description:Differences in basal leukocyte gene expression profiles in macaques classified as showing low, moderate, or high behavioral indications of perceived social isolation (PSI) Gene expression profiling was carried out on peripheral blood mononuclear cell RNA samples collected from 56 adult rhesus macaques classified as showing low, medium, or high behavioral indicators of PSI. Some analyses additionally controlled for age and RNA indicators of major leukocyte subsets (CD3E, CD3D, CD4, CD8A, CD19, FCGR3A/CD16, NCAM1/CD56, CD14).

2015-11-23 | E-GEOD-65243 | biostudies-arrayexpress

Transcription profiling of peripheral blood leukocyte subsets in autoimmune/autoinflammatory disease patients and healthy controls

Project description:Gene expression microarray profiling was performed on peripheral blood leukocyte subsets (CD4+ T cells, CD14+ monocytes, and CD16+ neutrophils) from healthy controls and patients with flaring autoimmune disease.

2014-08-31 | E-MTAB-2452 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data