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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq after knockdown of the endogeneous retrovirus HERVH in human embryonic stem cells

ABSTRACT: Human endogenous retrovirus subfamily H (HERVH) is a class of transposable elements whose members are expressed preferentially in human pluripotent stem cells. Here, we report that the long-terminal repeat regions of HERVH function as enhancers which are regulated by OCT4. Strikingly, we found that HERVH is a nuclear-localized long non-coding RNA that is required to maintain the undifferentiated state of human embryonic stem cells. Furthermore, we showed that HERVH is associated with OCT4 and co-activators such as P300, CBP and mediator subunits. Together, these results uncover a new and unexpected role of species-specific transposable elements in specifying the pluripotency of human cells.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Xinyi Lu

PROVIDER: E-MTAB-2072 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The retrovirus HERVH is a long noncoding RNA required for human embryonic stem cell identity.

Lu Xinyi X Sachs Friedrich F Ramsay LeeAnn L Jacques Pierre-Étienne PÉ Göke Jonathan J Bourque Guillaume G Ng Huck-Hui HH

Nature structural & molecular biology 20140330 4

Human endogenous retrovirus subfamily H (HERVH) is a class of transposable elements expressed preferentially in human embryonic stem cells (hESCs). Here, we report that the long terminal repeats of HERVH function as enhancers and that HERVH is a nuclear long noncoding RNA required to maintain hESC identity. Furthermore, HERVH is associated with OCT4, coactivators and Mediator subunits. Together, these results uncover a new role of species-specific transposable elements in hESCs. ...[more]

PMID: 24681886

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