Biological Impact Of Environmental Toxicants In Vitro

ABSTRACT: Exposure to cigarette smoke is a leading cause of lung diseases including chronic obstructive pulmonary disease and cancer. Cigarette smoke is a complex aerosol containing over 6000 chemicals, and thus it is difficult to determine individual contributions to overall toxicity, and the molecular mechanisms by which smoke constituents exert their effects. We selected three well-known harmful and potentially harmful constituents (HPHCs) in tobacco smoke: acrolein, formaldehyde and catechol and established a High Content Screening (HCS) method using normal human bronchial epithelial cells, which are the first bronchial cells in contact with cigarette smoke. The impact of each HPHC was investigated using 13 multi-parametric indicators of cellular toxicity and complemented with a microarray-based

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 7G

ORGANISM(S): Homo sapiens

SUBMITTER: Sam Ansari 

PROVIDER: E-MTAB-2080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress