VNO_MOE_microarrays
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Ximena Ibarra-Soria
PROVIDER: E-MTAB-2163 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:The Vomeronasal organ (VNO) is a part of the accessory olfactory system, which is responsible for detecting pheromones, chemical factors that trigger a spectrum of sexual and social behaviors. The vomeronasal epithelium (VNE) shares several features with the epithelium of the main olfactory epithelium (MOE). However, it is a distinct neuroepithelium populated by chemosensory neurons that differ from the olfactory sensory neurons (OSNs) in cellular structure, receptor expression, and connectivity. The vomeronasal organ of rodents comprises a sensory epithelium and a thin nonsensory epithelium that morphologically resembles the respiratory epithelium. Sox2-positive cells have been previously identified as the stem cell population that gives rise to neuronal progenitors in MOE and VNE. In addition to these, the MOE also comprises p63 positive horizontal basal cells (HBCs), a second pool of quiescent stem cells that become active in response to injury. Immunolabeling against the transcription factor p63, Keratin-5 (Krt5), Krt14 and Krt5Cre tracing experiments highlighted the existence of horizontal basal cells distributed along the basal lamina of the VNO forming from progenitors along the basal lamina oft the marginal zones. Moreover, these experiments revealed that the NSE of rodents is, like the respiratory epithelium, a stratified epithelium where the p63/Krt5+ basal cells self-replicate and give rise to the apical columnar cells facing the lumen of the VNO.
2024-03-11 | GSE247872 | GEO
Project description:High-throughput RNA sequencing of the olfactory system of newborn mice.With this study we aim to assess the expression profile of the main olfactory epithelium (MOE) and the vomeronasal organ (VNO) at birth in C57BL/6J mice. Tissue was collected from P0.5 pups and three to four individuals were pooled for each sample. Three biological replicates were obtained for each organ. RNA was extracted using the Qiagen RNeasy kit using standard protocols. All six RNA samples were multiplexed together and sequenced in two lanes on the Illumina HiSeq platform.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
2013-08-07 | E-ERAD-169 | biostudies-arrayexpress
Project description:Understanding the molecular mechanisms defining and maintaining the identity of a specific neuronal cell type is a central goal in neuroscience. The vomeronasal organ (VNO) of mice contains hundreds of distinct vomeronasal sensory neurons (VSNs). The VSNs are classified into two major cell types that are segregated in apical and basal regions of the VNO, express vomeronasal receptors of different superfamilies, and send axons to different portions of the accessory olfactory bulb. How apical or basal identity of VSNs is established and maintained is largely unknown. Here we attempt to assess the role of a single transcription factor, AP-2ε, in the VNO of mice. We used microarrays to examine global effect of AP-2ε loss-of-function on gene expression in the vomeronasal organ.
2018-02-03 | GSE110083 | GEO
Project description:We sought to investigate the scope of cellular and molecular changes within a mouse’s olfactory system as a function of its exposure to odors emitted from members of the opposite sex. To this end, we housed mice either separated from members of the opposite sex (sex-separated) or together with members of the opposite sex (sex-combined) until six months of age and then profiled transcript levels within the main olfactory epithelium (MOE), vomeronasal organ (VNO), and olfactory bulb (OB) of the mice via RNA-seq. For each tissue type, we then analyzed gene expression differences between sex-separated males and sex-separated females (SM v SF), sex-combined males and sex-combined females (CM v CF), sex-separated females and sex-combined females (SF v CF), and sex-separated males and sex-combined males (SM v CM). Within both the MOE and VNO, we observed significantly more numerous gene expression differences between males and females when mice were sex-separated as compared to sex-combined. Chemoreceptors were highly enriched among the genes differentially expressed between males and females in sex-separated conditions, and these expression differences were found to reflect differences in the abundance of the corresponding sensory neurons.
2018-11-01 | GSE112352 | GEO
Project description:We have generated single cell transcriptomic atlases of vomeronasal organs (VNO) from juvenile and adult mice. Combined with spatial molecular imaging, we uncover a distinct, previously unidentified class of cells that express the vomeronasal receptors and a population of canonical olfactory sensory neurons in the VNO. High resolution trajectory and cluster analyses reveal the lineage relationship, spatial distribution of cell types, and a putative cascade of molecular events that specify the V1r, V2r, and OR lineages from a common stem cell population. The expression of vomeronasal and olfactory receptors follow power law distributions, but there are high variabilities in average expression levels between individual receptor and cell types. Substantial co-expression is found between receptors across clades, from different classes, and between olfactory and vomeronasal receptors, with nearly half from pairs located on the same chromosome. Interestingly, the expression of V2r, but not V1r, genes is associated with various transcription factors, suggesting distinct mechanisms of receptor choice associated with the two cell types. We identify association between transcription factors, surface axon guidance molecules, and individual VRs, thereby uncovering a molecular code that guides the specification of the vomeronasal circuitry. Our study provides a wealth of data on the development and organization of the accessory olfactory system at both cellular and molecular levels to enable a deeper understanding of vomeronasal system function.
2024-02-29 | GSE252365 | GEO
Project description:The vomeronasal organ (VNO) of mice contains two main types of vomeronasal sensory neurons (VSNs)- Apical and Basal. Apical VSNs express vomeronasal receptors (VRs) of the V1R family and project to the anterior accessory olfactory bulb (AOB) and VSNs in the basal portions of the epithelium express receptors of the V2R family and project to the posterior portion of the AOB. In the vomeronasal epithelium of mice we found active BMP signaling. By generating Smad4 conditional mutants we disrupted canonical TGF-b/BMP signaling in either maturing basal VSNs or in mature apical and basal VSNs.
2019-07-19 | GSE134492 | GEO
Project description:Chemical signals are essential for communication between living organisms. Dogs possess two sensory organs enabling chemical communication; the main olfactory system and the vomeronasal organ (VNO). Besides, contact chemoreception is also pertinent, by which non-volatile molecules, including but not limited to proteins, are recognized as chemical signals. However, non-volatile chemical signals have been sparsely studied in dogs. Therefore, we aimed to examine the urinary proteins of female domestic dogs during estrus and anestrus phases to detect and identify such non-volatile chemical signals.
2023-05-10 | PXD040418 | Pride
Project description:To examine the impact of selective breeding for voluntary wheel running activity on gene expression in the vomeronasal organ (VNO), RNAseq analysis was conducted in the VNO of 4 High Runner and 4 Control lines.
2020-11-26 | GSE146644 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE39514: Effects of H2be ectopic over-expression on gene expression in the main olfactory epithelium (MOE) of 5-week old mice. GSE39515: Effects of H2be loss of function on gene expression in the main olfactory epithelium (MOE) of 6-month old mice. GSE39516: Effects of H2be loss of function on gene expression changes in the main olfactory epithelium (MOE) as a result of activity deprivation through unilateral naris occlusion (UNO). GSE39517: Genome-wide location analysis of FLAG-H2BE Refer to individual Series
2012-09-01 | E-GEOD-39674 | biostudies-arrayexpress
Project description:<1. The vomeronasal organ (VNO) in the nose of most tetrapods is a sensory structure for the detection of pheromones and kairomones (intra-specific and inter-specific chemical signals) that initiate innate behaviours. It has two neuronal layers, each expressing distinct receptor sub-families coupled to different G-proteins. Neurones in the apical layer express a single vomeronasal receptor type 1 and the G?i2 G-protein. On the other hand, neurons in the basal layer express two or more vomeronasal receptors type-2 (V2Rs) and the G?o G-protein. In mice, V2Rs are organized into four families, A, B, D and C, with family-A and -C sub-divided into subfamilies A1-A10 and C1-C2. These gene families are expressed in a unique combinatorial pattern. Families A (subfamilies A1-A6), B and D are expressed monogenically and coexpress with the recently expanded family-C2 (6 genes, Vmn2r2 to Vmn2r7). While neurons expressing the phylogenetically ancient subfamily-C2 (1 gene, Vmn2r1) coexpress with family-BD and subfamilies A8-A10.We have generated a knock-out mouse for gene Vmn2r1 gene, using a Eucomm tm1b allele. This mouse is a valuable resource for further understanding the molecular organization of the VNO and its behavioural implications, in an evolutionary context. We propose here, to sequence the VNO of Vmn2r1 mutant mice. By studying the VNO transcriptome we hope to advance our knowledge of the genetic coding of pheromonal communication. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
2015-10-20 | E-ERAD-402 | biostudies-arrayexpress