The Global Molecular Landscape of Hematopoietic Stem Cells and their Immediate Progeny

ABSTRACT: Hematopoietic stem cells (HSC) possess life-long self-renewal activity and generate a series of multipotent progenitors that differentiate into lineage-committed progenitors and subsequently mature cells. Recently, functionally distinct stem and progenitor cell types have been identified, however, a systems-wide understanding of the underlying gene regulation is lacking. Here, we present the global transcriptome of ex vivo isolated mouse multipotent hematopoietic stem cells (HSC) and multipotent progenitor cells (MPP1-MPP4) as revealed by next-generation sequencing (RNA-seq). A related high-throughput study with DNA methylome data was also conducted, with data deposited at NCBI Gene Expression Omnibus, under accession GSE52709 ( http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52709 ). An imported copy of the methylome data set can also be found at ArrayExpress: http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52709.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Alejandro Reyes 

PROVIDER: E-MTAB-2262 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress