Systems biology approaches reveal low-dose effects of nanoparticles

ABSTRACT: Here, we show that systems biology approaches can uncover mechanisms underlying cellular responses to nanomaterials. Using RNA Seq, we found that cationic nanoparticles are capable of triggering down-regulation of cell cycle-related genes in primary human bronchial epithelial cells at doses that do not elicit acute cytotoxicity. Bioinformatics analyses implicated NF-kappaB as a putative transcriptional regulator and functional assays confirmed that cationic nanoparticles caused NF-kappaB-dependent cell cycle arrest. Our study demonstrates the feasibility of applying systems biology tools to assess cellular responses to nanomaterials, not least at low doses.

INSTRUMENT(S): Illumina HiSeq 2500, Computater Servers UPPMAX-SNIC., NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE), Dynamic light scattering and zeta potential. The experiments were performed on a Malvern Zetasizer NanoZS instrument using a He-Ne laser (Max, 5 mW) at 633 nm., Illumina Tru-seq RNA

ORGANISM(S): Homo sapiens

SUBMITTER: Pekka Kohonen 

PROVIDER: E-MTAB-2397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress