Transcription profiling by array of transgenic Arabidopsis over-expressing GmDREB1 family genes with or without treatment of 1 uM dexamethazon
Ontology highlight
ABSTRACT: Total RNA was isolated from 18-day-old seedlings of the transgenic Arabidopsis plants grown on GM agar plates with or without treatment of 1 uM dexamethazon
Project description:NF-YC10 is a subunit of the NF-Y transcription factor complex in Arabidopsis thaliana. We identified NF-YC10 as an interactor of the transcription factor DREB2A, which binds to DRE and activate expression of target genes under heat and/or dehydration stress conditions. Transgenic Arabidopsis plants that overexpress NF-YC10 showed stronger expression of DREB2A target genes under heat stress and were more tolerant to heat stress tolerance than the vector control plants. We conducted a transcript profiling by microarray aiming to identify target genes of NF-YC10 under heat stress
Project description:HsfA1d is one of the three master regulators (HsfA1s; HsfA1a, b and d) in the heat stress response of Arabidopsis thaliana. The protein levels of these HsfA1s are steady before and after heat stress, suggesting the existence of post-translational regulation. Through analyses of a deletion series of the HsfA1d protein, we identified a regulatory domain in this protein. The deletion of this domain turned the HsfA1d protein into an active form, and its expression resulted in induction of heat stress-inducible genes at room temperature. This microarray experiment was conducted to identify genes that are regulated by the function of the newly identified regulatory domain of HsfA1d.
Project description:To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress two transcription factors: DREB1A and OsPIL1. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and OsPIL1 enhances cell elongation through activation of cell wall synthesis in rice. We performed microarray experiments using a single overexpressor of OsPIL1 and the double overexpressor of DREB1A and OsPIL1 to study the potential interactions of the gene networks regulated by the two transcription factors in the double overexpressor.
Project description:HSC70 is the cytosolic isoform of plant HSP70. We have found that HSC70 family proteins bind to the heat shock transcription factor A1s (HsfA1s), which are the master regulators of the heat shock response in plants, and supress their activity. To investigate the role of HSC70s in the regulation of HsfA1s and heat shock responses, we evaluated the effect of triple knock out of HSC70s on the transcriptome under the normal growth condition.
Project description:To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress two transcription factors: DREB1A and PIF4. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and PIF4 enhances cell elongation through activation of cell wall synthesis. We performed microarray experiments using a single overexpressor of PIF4 and the double overexpressor of DREB1A and PIF4 to study the potential interactions of the gene networks regulated by the two transcription factors in the double overexpressor.
Project description:To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress DREB1A and GA5. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and GA5 encodes a GA synthetic enzyme which enhances plant growth. We performed microarray experiments using a single overexpressor of GA5 and the double overexpressor of DREB1A and GA5 to study the potential interactions of the gene networks regulated by the two factors in the double overexpressor.
Project description:HSC70 is the cytosolic isoform of plant HSP70. We have found that HSC70 family proteins bind to the heat shock transcription factor A1s (HsfA1s), which are the master regulators of the heat shock response in plants, and suppress their activity. We additionally found that the triple knock out of HSC70s alters responses of Arabidopsis plants to salt stress. To investigate the role of the HSC70s in salt stress responses, we evaluated the effects of the triple knock out on the transcriptome under salt stress.
Project description:BPMs are a family of E3 ubiquitin ligases. We identified BPM proteins as interactors of the Arabidopsis stress responsive transcription factor DREB2A. We found an increase in the accumulation level of the DREB2A protein under heat stress in transgenic Arabidopsis plants in which expression of six BPMs were suppressed by an amiRNA. In order to evaluate the impacts of the amiRNA on the gene expression under heat stress, transcriptomes were compared between these transgenic plants and vector control plants.