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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Th single cell RNA-Seq

ABSTRACT: Th2 cells regulate helminth infections, allergic disorders, tumor immunity and pregnancy by secreting various cytokines. It is likely that there are undiscovered Th2-signaling molecules. While steroids are known to be immunoregulators, de novo steroid production from immune cells has not been previously characterised. We demonstrate production of the steroid pregnenolone by Th2 cells in vitro, and in vivo in a helminth infection model. To identify gene-expression identify of these steroidogenic-Th2 cells, we performed single-cell RNA-sequencing.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Teichmann

PROVIDER: E-MTAB-2512 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis.

Mahata Bidesh B Zhang Xiuwei X Kolodziejczyk Aleksandra A AA Proserpio Valentina V Haim-Vilmovsky Liora L Taylor Angela E AE Hebenstreit Daniel D Dingler Felix A FA Moignard Victoria V Göttgens Berthold B Arlt Wiebke W McKenzie Andrew N J AN Teichmann Sarah A SA

Cell reports 20140510 4

T helper 2 (Th2) cells regulate helminth infections, allergic disorders, tumor immunity, and pregnancy by secreting various cytokines. It is likely that there are undiscovered Th2 signaling molecules. Although steroids are known to be immunoregulators, de novo steroid production from immune cells has not been previously characterized. Here, we demonstrate production of the steroid pregnenolone by Th2 cells in vitro and in vivo in a helminth infection model. Single-cell RNA sequencing and quantit ...[more]

PMID: 24813893

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