RBM20 orchestrates cardiac pre-mRNA

ABSTRACT: The RNA-binding protein RBM20 has been implicated in dilated cardiomyopathy (DCM), a major cause of chronic heart failure. To determine how RBM20 regulates alternative splicing, we combined transcriptome-wide CLIP-seq, RNA-seq, and quantitative proteomics in cell culture, rat, and human hearts. Our analyses revealed a distinct RBM20 RNA-recognition element in predominantly intronic binding sites and linked repression of exon splicing with RBM20-binding near 3prime- and 5prime-splice sites. Our proteomic data show RBM20 interaction with U1- and U2-snRNPs and suggests splicing repression through spliceosome stalling at complex A. Among direct RBM20 targets are several genes involved in DCM as well as new genes not previously associated with the disease process. In human failing hearts, we de

INSTRUMENT(S): Illumina Genome Analyzer II, Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Oliver Hummel 

PROVIDER: E-MTAB-2572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress