MicroRNA expression profiling of urine and cancer tissues of patients with bladder cancer, and those from the controls with haematuria

ABSTRACT: Mid-stream urine was collected from bladder cancer patients prior to surgery. Both tumor tissue and normal bladder mucosa that are located at >3cm away from the tumor edge were obtained by cystoscopy. For the normal controls with haematuria, urine samples were collected from patients who had normal cystoscopic finding and absence of malignancy with >6 months follow-up. All urine samples were centrifuged at 2500 r.c.f. for 20 minutes and the urine supernatant was collected. Total RNA of urine supernatant and frozen tissue was extracted using MirVanaTM PARISTM Kit (Ambion) in accordance with the manufacturer’s recommended protocols. AgilentTM Human miRNA Microarray Chip (Release 13.0, Agilent Technologies, Santa Clara, CA, USA) was used to determine the microRNA expression profiles of the samples.

INSTRUMENT(S): Agilent Microarray Hybridization Chamber Kit and Hybridization oven rotator, Agilent microarray Scanner, Agilent miRNA Complete Labeling and Hyb Kit, Extraction kit

ORGANISM(S): Homo sapiens

SUBMITTER: CF Ng 

PROVIDER: E-MTAB-2573 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress