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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Affymetrix MOE430v2 vs Mouse PancChip 5.0 comparison

ABSTRACT: Comparison of two microarray platforms: the Mouse PancChip 5.0 and the Affymetrix GeneChip Mouse Genome 430 2.0 Array. The aim of this experiment was to determine the ability to identify differentially expressed genes in islet and pancreas RNA, and the sensitivity of the two platforms, using the same source material in a carefully controlled manner. RNA was extracted from adult mouse pancreas (n=5) and highly purified islet samples (n = 5). All samples were amplified once. 5 biological replicates (islets vs. pancreas) in a dye swap experimental design were hybridized to the PancChip. 3 biological replicates, using the same amplified RNA hybridized to the PancChip, of both the pancreas and islet samples were also hybridized to the GeneChip. All data were normalized using appropriate methods and differential expression between islet and pancreas was determined using PaGE with a 10% FDR. The study revealed that the PancChip is a highly cost effective alternative to the Affymetrix 430-2, that the PancChip is up to 60% more sensitive than the Affymetrix 430-2 GeneChip and that 80% (7,000) of the probe sets on the PancChip show expression in either Islets or Pancreas, while only 25% (6,800) show expression on the Affymetrix GeneChip.

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 (GCS3000), Agilent DNA Microarray Scanner Model #G2565BA, Affymetrix Fluidics Station 400

ORGANISM(S): Mus musculus

SUBMITTER: Klaus Kaestner

PROVIDER: E-MTAB-2715 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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