Affymetrix MOE430v2 vs Mouse PancChip 5.0 comparison

ABSTRACT: Comparison of two microarray platforms: the Mouse PancChip 5.0 and the Affymetrix GeneChip Mouse Genome 430 2.0 Array. The aim of this experiment was to determine the ability to identify differentially expressed genes in islet and pancreas RNA, and the sensitivity of the two platforms, using the same source material in a carefully controlled manner. RNA was extracted from adult mouse pancreas (n=5) and highly purified islet samples (n = 5). All samples were amplified once. 5 biological replicates (islets vs. pancreas) in a dye swap experimental design were hybridized to the PancChip. 3 biological replicates, using the same amplified RNA hybridized to the PancChip, of both the pancreas and islet samples were also hybridized to the GeneChip. All data were normalized using appropriate methods

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 (GCS3000), Agilent DNA Microarray Scanner Model #G2565BA, Affymetrix Fluidics Station 400

ORGANISM(S): Mus musculus

SUBMITTER: Klaus Kaestner 

PROVIDER: E-MTAB-2715 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress