Transcriptome-wide analysis of mRNA decay in normal and osteoarthritic human articular chondrocytes

ABSTRACT: Human articular chondrocytes were isolated from normal or osteoarthritic tissue. RNA decay was measured across the transcriptome in these cells by microarray analysis following an actinomycin D chase for 0, 1, 3 and 5 hours. Normalisation was conducted by quantile normalising each set of four decay curve points (i.e. 0, 1, 3 and 5 hour samples for a given donor's cells) independently of the other data. This meant that each decay curve is normalised independently of the others.

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Tew 

PROVIDER: E-MTAB-2723 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress