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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of mouse pancreatic beta cells of MIN6 insulinoma cell line transfected with siRNA directed against murine Meis3 or non targeting control.

ABSTRACT: In order to identify genes that are regulated by Meis3, MIN6 insulinoma cells were transfected with siRNA directed against murine Meis3 (siMeis3) or non targeting control (siControl). RNA was harvested and reverse transcribed. Reduced expression of Meis3 was confirmed. The RNA was then submitted for labeling and hybridization to the Agilent cDNA microarray.

ORGANISM(S): Mus musculus

SUBMITTER: David Groff

PROVIDER: E-MTAB-273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Three-amino-acid-loop-extension homeodomain factor Meis3 regulates cell survival via PDK1.

Liu Jiangying J Wang You Y Birnbaum Morris J MJ Stoffers Doris A DA

Proceedings of the National Academy of Sciences of the United States of America 20101108 47

Three-amino-acid-loop-extension (TALE) homeodomain proteins including Meis and Pbx families are generally recognized for their roles in growth and differentiation during vertebrate embryogenesis and tumorigenesis. Whereas genetic studies indicate that Pbx1 regulates the development and function of insulin-producing pancreatic β-cells, the role of Meis family members in β-cells is still unknown. Here we show that Meis3 is abundantly expressed in pancreatic islets and β-cells and that it regulates ...[more]

PMID: 21059917

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