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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of neonatal and adult rat ventricular cardiomyocytes

ABSTRACT: We addressed the question of which protein kinases are expressed in cardiomyocytes. We assessed the changes during postnatal development, comparing profiles in rat neonatal ventricular cardiomyocytes (NVMs) with adult ventricular cardiomyocytes (AVMs). Neonatal and adult rat ventricular cardiomyocytes prepared according to established procedures (Marshall et al. PLoS ONE 2010 5(4):e10027; Fuller and Sugden, FEBs Lett. 1989 247:209-12; Rodrigues and Severson In Biochemical Techniques in the Heart (McNeill, J. H., Ed.) pp 101-115, CRC Press, New York.). mRNA expression profiles compared using Affymetrix rat genome 230 2.0 arrays.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Angela Clerk

PROVIDER: E-MTAB-2832 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cardiac protein kinases: the cardiomyocyte kinome and differential kinase expression in human failing hearts.

Fuller Stephen J SJ Osborne Sally A SA Leonard Sam J SJ Hardyman Michelle A MA Vaniotis George G Allen Bruce G BG Sugden Peter H PH Clerk Angela A

Cardiovascular research 20150810 1

<h4>Aims</h4>Protein kinases are potential therapeutic targets for heart failure, but most studies of cardiac protein kinases derive from other systems, an approach that fails to account for specific kinases expressed in the heart and the contractile cardiomyocytes. We aimed to define the cardiomyocyte kinome (i.e. the protein kinases expressed in cardiomyocytes) and identify kinases with altered expression in human failing hearts.<h4>Methods and results</h4>Expression profiling (Affymetrix micr ...[more]

PMID: 26260799

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