Collagen induces maturation of human monocyte-derived dendritic cells by signalling through Osteoclast Associated Receptor

ABSTRACT: Human immature dendritic cells derived from monocytes were activated to become mature dendritic cells by exposure to platebound Collagen I or Collagen. The importance of OSCAR-signalling was evaluated by pre-incubating the immature dendritic cells with an inhibitory monoclonal antibody to the OSCAR receptor, or an isotype control. As a positive control, immature dendritic cells were activated by using LPS. The set-up was examined at 4 hours and 20 hours to evaluate early and late effects of OSCAR signalling.

INSTRUMENT(S): NanoDrop® ND-1000 (Saveen&Werner)., Affymetix Gene Titan, Affymetrix Gene Titan, Agilent 2100 Bioanalyser, RNA 6000 Nano Kit chips (Agilent Technologies). NanoDrop® ND-1000 (Saveen&Werner).

ORGANISM(S): Homo sapiens

SUBMITTER: Pernille Keller 

PROVIDER: E-MTAB-2904 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress