Differential DNA methylation profiles of coding and non-coding genes define hippocampal sclerosis in human temporal lobe epilepsy - miRNA

ABSTRACT: Methylation profiling was carried using Methylated DNA immunoprecipitation coupled with microarray consisting of custom designed Nimblegen arrays with tiled regions 50kb upstream and 20kb downstream of all 754 miRNAs for human tissue samples. The samples, i.e. immunoprecipitate & input were deferentially labelled using Cy5 & Cy3 flourophores respectively. These were subsequently hybridized on the arrays and thereafter washed and scanned. The samples used for this study - Hippocampal tissue was scored according to the Watson hippocampal sclerosis grading system- Watson Grade 1 or 4. Control tissue (labelled as Control in disease staging) was obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD.

INSTRUMENT(S): Scanning of was performed on an Axon 4000B scanner

ORGANISM(S): Homo sapiens

SUBMITTER: Sudipto Das 

PROVIDER: E-MTAB-3055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress