Early gene expression in Pseudomonas fluorescens exposed to a polymetallic solution
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ABSTRACT: Our main objective was to study the changes in cDNA microarray gene expression profiles of P. fluorescens cells exposed to different doses of a polymetallic solution containing Ag (I), Pb (II), Cd (II), Cu (II), Ni (II) and Zn (II)) over two exposure times (5 and 15 minutes). Control cells grown in the absence of metals were also included in the experiment.
Project description:Our main objective was to study the changes in cDNA microarray gene expression profiles of E. coli cells exposed to different doses of a polymetallic solution containing Ag (I), Pb (II), Cd (II), Cu (II), Ni (II) and Zn (II)) over three exposure times (5, 10 and 15 minutes). Control cells grown in the absence of metals were also included in the experiment.
Project description:Our main objective was to study the changes in cDNA microarray gene expression profiles of A. thaliana plants exposed to different doses of a polymetallic solution containing Pb (II), Hg (II), Cu (II), Cd (II), Co (II), Ni (II), Zn (II) and Mn (II) over 3 hours. Control plants grown in the absence of metals were also included in the experiment.
Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(II) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media Two-condition experiment, iron(II) chloride supplemented culture versus non-iron treated culture. 4 biological replicates including 3 technical replicates for one of the biological replicates. Swap-dye experiments were performed
Project description:This SuperSeries is composed of the following subset Series: GSE29319: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(II) chloride GSE29320: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(III) chloride Refer to individual Series
Project description:In this study, we examined the gas-phase redox chemistry of the [copper(II) – amyloid β] complex. Our findings reveal that the sequence-dependent variations in this chemistry correspond to significant aspects of the in vitro behavior of different peptide variants.
Project description:The present work is the pioneering report depicting the total proteomic atlas of four major developmental stages (third, fourth, fifth instar and pupa) of M. vitrata by LC-MS/MS analyses.
Project description:We have immunoprecipitated MHC class-I and class-II complexes from THP-1 macrophages infected with BCG, eluted MHC-bound peptides and analysed them by liquid chromatography tandem mass spectrometry.
Project description:Cupriavidus metallidurans CH34 is a metal resistant beta-proteobacterium. The genome of this bacterium contain many genes involved in heavy metal resistance. Gene expression of C. metallidurans was studied after the addition of of Zn(II), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II) or Co(II). Keywords: Heavy metal stress response Cultures of C. metallidurans CH34 were grown at 30°C until OD reached 0.6 (mid- exponential phase cultures). Heavy metals (0.8 mM of Zn(II), 0.5 mM of Cd(II), 0.1 mM of Cu(II), 0.6 mM of Ni(II), 0.4 mM of Pb(II), 5 uM of Hg(II) and 0.5 mM of Co(II)) were added to the culture for 30 minutes induction time. Total RNA was extracted, reverse-transcribed and labeled with Cy3-dCTP for the control (without metal) and with Cy5-dCTP for each conditions (challenged with one metal). Labeled cDNA were (control and one condition) added to a spotted slide for overnight hybridization at 42°C. Slides were scanned with a laser at 532 and 635 nm.
Project description:Sequencing by the Illumina Genome Analyzer II(36 bp) of the reverse transcribed small RNAs (<500b) using the Illumina directional mRNA-Seq protocol. This was performed on A. fabrum C58 strain from several growth conditions. Reads were then aligned to the genome of the bacteria (Accession number NC_003062, NC_003063, NC_003064 and NC_003065)