Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effects of low or high concentrations of monothioglycerol on human embryonic stem cell derived EBs

ABSTRACT: Prior studies indicated that high concentrations of monothioglycerol (MTG), a thio-containing compound, is necessary to promote the survival or differentiation of insulin 1 expressing cells from murine ES-derived embryonic bodies (EBs). In this experiment, we tested the hypothesis that human ES cell-derived EBs, when cultured in the presence of high concentrations of MTG could preferentially differentiate to definitive endoderm, compared to those cultured in the presence of low concentrations of MTG. In addition, we wish to establish that the EBs cultured from the high MTG group is effectively committed to all 3 germ layers, compared to the undifferentiated ES cells. Thus, 3 groups of samples were submitted for micro-array analyses. Each group has 4 biological replicates.

INSTRUMENT(S): Agilent DNA Microarray Scanner Model #G2565BA

ORGANISM(S): Homo sapiens

SUBMITTER: Teresa Ku

PROVIDER: E-MTAB-3124 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes1-4. In plants, timely transition to a flowering state is crucial for successful reproduction5-7. EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis8,9. Here, we revealed that bivalent bromo-adjacent homology (BAH)-plant homeodomain (PHD) reader modules of EBS bind H3K27me3 and H3K4me3, respectively. A subset of EBS-associated genes was co-enriched with H3K4me3, H3K27me3, and the Polycomb repressor complex 2 (PRC2). Interestingly, EBS adopts an auto-inhibition mode to mediate its binding preference switch between H3K27me3 and H3K4me3. This binding balance is critical because disruption of either EBS-H3K27me3 or EBS-H3K4me3 interaction induces EBS-mediated early floral transition. This study identifies a single bivalent chromatin reader capable of recognizing two antagonistic histone marks and reveals a distinct mechanism of interplay between active and repressive chromatin states.The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes1-4. In plants, timely transition to a flowering state is crucial for successful reproduction5-7. EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis8,9. Here, we revealed that bivalent bromo-adjacent homology (BAH)-plant homeodomain (PHD) reader modules of EBS bind H3K27me3 and H3K4me3, respectively. A subset of EBS-associated genes was co-enriched with H3K4me3, H3K27me3, and the Polycomb repressor complex 2 (PRC2). Interestingly, EBS adopts an auto-inhibition mode to mediate its binding preference switch between H3K27me3 and H3K4me3. This binding balance is critical because disruption of either EBS-H3K27me3 or EBS-H3K4me3 interaction induces EBS-mediated early floral transition. This study identifies a single bivalent chromatin reader capable of recognizing two antagonistic histone marks and reveals a distinct mechanism of interplay between active and repressive chromatin states.v

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Effects of low or high concentrations of monothioglycerol on human embryonic stem cell derived EBs

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