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ChIP-Chip and SACO Analysis of Pdx-1 binding in NIT-1 Insulinoma Cells

ABSTRACT: The BCBC Promoter Chip 5B was used to identify genomic targets of Pdx-1 binding. Genomic DNA from mouse NIT-1 insulinoma cells was immunoprecipitated with a Pdx-1 Antibody, PCR amplified, and labeled to the chip. All hybridizations were versus a common reference sample which was a labeled IgG IP. Additionally a Pdx1 SACO library for NIT-1 cells was generated.

INSTRUMENT(S): Agilent DNA Microarray Scanner Model #G2565BA

ORGANISM(S): Mus musculus

SUBMITTER: Richard Goodman

PROVIDER: E-MTAB-3126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterization of pancreatic transcription factor Pdx-1 binding sites using promoter microarray and serial analysis of chromatin occupancy.

Keller David M DM McWeeney Shannon S Arsenlis Athanasios A Drouin Jacques J Wright Christopher V E CV Wang Haiyan H Wollheim Claes B CB White Peter P Kaestner Klaus H KH Goodman Richard H RH

The Journal of biological chemistry 20070830 44

The homeobox transcription factor Pdx-1 is necessary for pancreas organogenesis and beta cell function, however, most Pdx-1-regulated genes are unknown. To further the understanding of Pdx-1 in beta cell biology, we have characterized its genomic targets in NIT-1 cells, a mouse insulinoma cell line. To identify novel targets, we developed a microarray that includes traditional promoters as well as non-coding conserved elements, micro-RNAs, and elements identified through an unbiased approach ter ...[more]

PMID: 17761679

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