Chromatin immunoprecipitation of adult mouse liver to identify Foxa2 targets

ABSTRACT: We performed genome-wide location analysis for Foxa2 to identify its targets in the adult mouse liver. Chromatin isolated from the liver of five adult mice was cross-linked, sheared and immunoprecipitated with a Foxa2-specific antibody. The resulting material, as well as material that was not immunoprecipitated, was uncross-linked, amplified, labeled and hybridized to the Mouse Promoter Chip BCBC-5A. Statistical analysis resulted in a set of 107 genes that are bound by Foxa2. Using computational analyses, we showed that Foxa2 containing cis-regulatory modules are dependent on the strength of the Foxa2 consensus site present.

ORGANISM(S): Mus musculus

SUBMITTER: Klaus Kaestner 

PROVIDER: E-MTAB-32 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress