Spontaneous cell competition in U2OS human osteosarcoma cells.

ABSTRACT: The study compares the transcriptional profiles of U2OS human osteosarcoma cells and those of two single-cell derived sub-lines carrying a stably integrated H2BmCherry (U2OS-R1) or H2B-YFP (U2OS-YFP) expression cassette. U2OS-R1 and U2OS-YFP cell spontaneously undergo apoptosis upon co-culture with the parental (Wt) U2OS cell population. Furthermore, YFP cells also undergo apoptosis upon co-culture with R1 cells. This behavior is similar to the \cell competition\ phenomenon described in the Drosophila imaginal disc and the mouse epiblast (for review, see , where fast-growing \fit\ cells induce apoptosis in slow-growing, less \fit\ neighbors. The mRNA expression profiles of Wt, R1 and YFP cells cultured alone or in pairwise combinations for 48h were analyzed after cell sorting by RNA micro

ORGANISM(S): Homo sapiens

SUBMITTER: Alfredo Penzo Mendez 

PROVIDER: E-MTAB-3204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress