Testis-expressed cluster of microRNAs 959-964 controls spermatid differentiation in Drosophila melanogaster

ABSTRACT: MicroRNAs are a wide class of ~22 nt non-coding RNAs of metazoans capable of inhibiting target mRNAs translation by binding to partially complementary sites in their 3’UTRs. Due to their regulatory potential, miRNAs are implicated in functioning of a broad range of biological pathways and processes. Here we investigate the functions of the miR-959-964 cluster expressed predominantly in testes of Drosophila melanogaster. The deletion of miR-959-964 resulted in male sterility due to the disturbance of the spermatid individualization process. Analysis of the transcriptome by microarray followed by luciferase reporter assay revealed didum, for, fdl and CG10512 as the targets of miR-959-964. Moreover, the deletion of miR-959-964 is accompanied by a decreased the expression of genes responsibl

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sergei Ryazansky 

PROVIDER: E-MTAB-3214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress