Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of antigen-specific T cells upon CCR9 knockdown in MCF7 cells.


ABSTRACT: Tumor cells are known to express immune-inhibitory ligands on their surface to mediate resistance against cytotoxic T lymphocytes. Using a high-throughput RNAi screening assay, we identified CCR9 as a potent tumor-specific immune checkpoint molecule that inhibited T cell-mediated tumor lysis. To understand the mode of CCR9-mediated immune resistance, MCF7 tumor cells were transfected with control or CCR9-specific siRNAs for 72 hours and co-cultured with survivin antigen-specific T cells for an additional 12 hours. T cells were then purified from the co-culture and total RNA was isolated for gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Nisit Khandelwal 

PROVIDER: E-MTAB-3244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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