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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells

ABSTRACT: To gain more information about EMT induced by oncogenic Ras in MDCK cells, we performed RNA-seq of MDCK and MDCK-Ras cells and compared their genome-wide gene expression profiles.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Sabine Macho-maschler

PROVIDER: E-MTAB-3301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High-throughput mRNA and miRNA profiling of epithelial-mesenchymal transition in MDCK cells.

Shukla Priyank P Vogl Claus C Wallner Barbara B Rigler Doris D Müller Mathias M Macho-Maschler Sabine S

BMC genomics 20151116

<h4>Background</h4>Epithelial-mesenchymal transition (EMT) is an important process in embryonic development, especially during gastrulation and organ formation. Furthermore EMT is widely observed in pathological conditions, e.g., fibrosis, tumor progression and metastasis. Madin-Darby Canine Kidney (MDCK) cells are widely used for studies of EMT and epithelial plasticity. MDCK cells show an epithelial phenotype, while oncogenic Ras-transformed MDCK (MDCK-Ras) cells undergo EMT and show a mesench ...[more]

PMID: 26572553

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