ChIP-seq of E. coli to identify H-NS and Fis binding at multiple time-points during growth in rich medium

ABSTRACT: Among the most important regulators of gene expression in bacteria are 'nucleoid-associated proteins'. These proteins alter the topology of the bound DNA by bending, wrapping or bridging it, thus having multiple effects, including transcriptional regulation, on the bacterial cell. Among the best-studied nucleoid proteins are H-NS and Fis, which bind to specific sequences on the DNA. H-NS is a global repressor of gene expression. Fis alters the global conformation of the DNA by introducing branched structures in it; but its effect on gene expression on a genomic scale remains largely unclear.

Several bacterial transcriptional regulators including H-NS and Fis have been studied using ChIP-chip. However, the higher resolution and dynamic range offered by ChIP-Seq have not been exploite

ORGANISM(S): Escherichia coli

SUBMITTER: Aswin Sai Narain Seshasayee 

PROVIDER: E-MTAB-332 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress