ChIP-chip by array of human cells for whole genome profiling of 4 histone modifications

ABSTRACT: Whole genome profiling of 4 histone modifications (H3K27me1, H3K27me3,H3K36me1 and H3K36me3) using ChIP-on-chip. It has recently been shown that nucleosome distribution, histone modifications and RNA polymerase II (Pol II) occupancy show preferential association with exons ("exon-intron marking"), linking chromatin structure and function to co- transcriptional splicing in a variety of eukaryotes. Previous ChIP-sequencing studies suggested that these marking patterns reflect the nucleosomal landscape. By analyzing ChIP-chip datasets across the human genome in three cell types, we have found that this marking system is far more complex than previously observed. We show here that a range of histone modifications and Pol II are preferentially associated with exons. However, there is noticeable

ORGANISM(S): Homo sapiens

DISEASE(S): chronic myeloid leukemia

SUBMITTER: David Vetrie 

PROVIDER: E-MTAB-336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress