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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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P53 delta30 mutant

ABSTRACT: H1299 cells expressing tetracycline regulated (“tet-off”) delta30 p53 (delta364-393) were previously described (Chen et al., 1996; McKinney et al., 2004).

ORGANISM(S): Homo sapiens

SUBMITTER: Idit Shiff

PROVIDER: E-MTAB-3362 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The p53 C terminus controls site-specific DNA binding and promotes structural changes within the central DNA binding domain.

Laptenko Oleg O Shiff Idit I Freed-Pastor Will W Zupnick Andrew A Mattia Melissa M Freulich Ella E Shamir Inbal I Kadouri Noam N Kahan Tamar T Manfredi James J Simon Itamar I Prives Carol C

Molecular cell 20150301 6

DNA binding by numerous transcription factors including the p53 tumor suppressor protein constitutes a vital early step in transcriptional activation. While the role of the central core DNA binding domain (DBD) of p53 in site-specific DNA binding has been established, the contribution of the sequence-independent C-terminal domain (CTD) is still not well understood. We investigated the DNA-binding properties of a series of p53 CTD variants using a combination of in vitro biochemical analyses and ...[more]

PMID: 25794615

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