Transcription profiling by array of fetal and adult normal human pancreas tissue
Ontology highlight
ABSTRACT: Pooled total RNA from normal adult human pancreas and fetal human pancreas were purchased from a commercial vendor (Clontech). Each pooled sample was independently labeled and hybridized 3 times.
Project description:PaTu-8988s and PaTu-8988t are two cell lines which were derived from the same liver metastasis of a human primary pancreatic adenocarcinoma, but differ in their differentiation status and metastatic capacity. The poorly differentiated cell line PaTu-8988t was transfected with an E-Cadherin expression construct or vector control, respectively, and individual clones analyzed by expression profiling. In addition, 3 independent preparations of wild-type PaTu-8988s cells were analyzed.
Project description:Pancreatic cancer cells are inherently highly resistant to spontaneous or chemotherapy-induced apoptosis. The human pancreatic tumor cell line Capan-1 (pRB+/p16-) was stably transfected with p16 expression constructs or control plasmids to functionally inactivate pRB. Three independent experiments were conducted
Project description:The acute promyelocytic leukemia-derived cell line, NB4, was grown at 37°C in 5% CO2 in an RPMI medium supplemented with 2 mM L-glutamine and 10% decomplemented fetal calf serum. Cells were cultured for 48 h with or without 1 μM All-trans retinoic acid (ATRA). Duplicates of 3 independent experiments were analyzed.
Project description:The pancreatic cancer cell line A818-1 was grown either in 2D monolayers or in 3-dimensional “hollow spheres” as described in Lehnert et al. (1999), Ann N Y Acad Sci 880:83-93. Total RNA was extracted and analyzed from 3 independent experiments.
Project description:Sphingosylphosphorylcholine (SPC) is a bioactive lipid with multiple biological roles including the regulation of differentiation in embryonic and cancer cells. PaTu II pancreatic cancer cells were incubated with 15 μM SPC in serum-free Medium for 2 h or left untreated. Duplicates of 3 independent experiments were analyzed.
Project description:In brief, PANC-1 cells stably transfected with a dominant negative HRAS(S17N) mutant, a constitutively active KRAS2(G12V) mutant, or mock transfected with an EGFP expression construct were treated with TGFB1 or left untreated, respectively. Three independent experiments each were analyzed in duplicate.
Project description:S2-007 and S2-028 are cell lines derived from the same primary pancreatic adenocarcinoma, but displying strong differences in their invasive and metastatic potential both in vitro and in vivo. Three independent preparations each of the highly metastatic S2-007 and the low metastatic S2-028 cell line grown in 2D cell culture (DMEM (Gibco, Invitrogen, Carlsbad, CA) supplemented with 10% (v/v) fetal bovine serum (PAA, Pasching, Austria) in a humidified atmosphere and 5% CO2, 95% air at 37°C) were analyzed by expression profiling.
Project description:A total of 16 clinical samples from ductal adenocarcinoma, 6 samples from chronic pancreatitis, and 4 samples from morphologically normal resection margins from chronic pancreatitis resectates were analyzed.
Project description:PolyA+ RNA samples from the twelve healthy human tissues were purchased from Clontech (Palo Alto, CA). Each RNA sample is typically composed of a pool of 10-25 individuals. The expression intensity of mRNA was assayed across five microarrays (Affymetrix GeneChips U95A–E). Please note GeneNote is now retired: https://genecards.weizmann.ac.il/cgi-bin/genenote/home_page.pl. This dataset is part of the TransQST collection.
Project description:Constitutive Kras and NF-kB activation is identified as signature alterations in human pancreatic ductal adenocarcinoma (PDAC). Here, we report that pancreas-targeted IKK2/beta inactivation inhibited NF-kB activation and completely suppressed PDAC development. Our findings demonstrated that NF-kB is required for development of pancreatic ductal adenocarcinoma that was initiated by Kras activation. Pancreatic tissue from 4 groups of mice were used in this project: (1) the pancreas normal appearance of Pdx1-cre;KrasLSL-G12D;IKK2/beta mice, (2) the normal pancreas of Pdx1-cre;KrasLSL-G12D mice, (3) the pancreatic lesion of pancreatic intraepithelial neoplasia (PanIN) of Pdx1-cre;KrasLSL-G12D mice, and (4) the pancreatic lesion of PDAC of Pdx1-cre;KrasLSL-G12D mice. Each group included three mice. RNA samples from mouse pancreas were hybridized on GeneChip Mouse Gene 1.0 ST arrays (Affymetrix). Group (1) and group (2) were compared, and group (2), group (3) and group (4) were compared.